rat pituitary tumor cell line gh3  (ATCC)

Journal: Frontiers in Pharmacology

Article Title: Wogonin inhibits the proliferation of prolactinoma through the PI3K/AKT signaling pathway

doi: 10.3389/fphar.2025.1546285

Figure Lengend Snippet: Effects of wogonin on cell viability and colony formation: GH3 cell (A) and MMQ cell (B) survival was determined by the CCK-8 assay (C) . GH3 cells were treated with wogonin at concentrations of 0, 30, 60 and 120 μM (D) . The statistical graph of the result of the colony formation. (****p < 0.0001, ***p < 0.005, **p < 0.01, *p < 0.05, Except for * on the n-zig-zag line, all other * indicate comparisons with the control group).

Article Snippet: Rat pituitary tumor cell lines MMQ and GH3 were procured from Wuhan Procell Life Science and Technology Co., Ltd. (Wuhan, China).

Techniques: CCK-8 Assay, Control

Journal: Frontiers in Pharmacology

Article Title: Wogonin inhibits the proliferation of prolactinoma through the PI3K/AKT signaling pathway

doi: 10.3389/fphar.2025.1546285

Figure Lengend Snippet: Protein expression levels of p-PI3K and p-AKT expression in prolactinoma cells were detected by Western blot. GAPDH was used as an internal control. n = 5/group: (A) . GH3 cells were treated with wogonin at concentrations of 0, 30, 60 and 120 μM for 72 h (B) . Statistical graph of Western blot results of GH3 cells (C) . MMQ cells were treated with wogonin at concentrations of 0, 50, 100 and 200 μM for 72 h (D) . Statistical graph of Western blot results of MMQ cells. (**p < 0.01, *p < 0.05. Except for * on the n-zig-zag line, all other * indicate comparisons with the control group).

Article Snippet: Rat pituitary tumor cell lines MMQ and GH3 were procured from Wuhan Procell Life Science and Technology Co., Ltd. (Wuhan, China).

Techniques: Expressing, Western Blot, Control

Journal: Frontiers in Pharmacology

Article Title: Wogonin inhibits the proliferation of prolactinoma through the PI3K/AKT signaling pathway

doi: 10.3389/fphar.2025.1546285

Figure Lengend Snippet: Protein expression levels of Bax, cleaved caspase-9 and cleaved caspase-3 expression in prolactinoma cells were detected by Western blot. GAPDH was used as an internal control. n = 5/group: (A) . GH3 cells were treated with wogonin at concentrations of 0, 30, 60 and 120 μM for 72 h (B) . Statistical graph of Western blot results of GH3 cells (C) . MMQ cells were treated with wogonin at concentrations of 0, 50, 100 and 200 μM for 72 h (D) . Statistical graph of Western blot results of MMQ cells. (**p < 0.01, *p < 0.05, Except for * on the n-zig-zag line, all other * indicate comparisons with the control group).

Article Snippet: Rat pituitary tumor cell lines MMQ and GH3 were procured from Wuhan Procell Life Science and Technology Co., Ltd. (Wuhan, China).

Techniques: Expressing, Western Blot, Control

Journal: Frontiers in Pharmacology

Article Title: Wogonin inhibits the proliferation of prolactinoma through the PI3K/AKT signaling pathway

doi: 10.3389/fphar.2025.1546285

Figure Lengend Snippet: Levels of PRL secretion under wogonin treatment in prolactinoma cells: (A) . GH3 cells were treated with wogonin at concentrations of 0, 30, 60 and 120 μM for 72 h (B) . MMQ cells were treated with wogonin at concentrations of 0, 50, 100 and 200 μM for 72 h (*p < 0.05, all * indicate comparisons with the control group, ns means no significance).

Article Snippet: Rat pituitary tumor cell lines MMQ and GH3 were procured from Wuhan Procell Life Science and Technology Co., Ltd. (Wuhan, China).

Techniques: Control